quick dna viral kit zymo research cat (Zymo Research)

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Zymo Research quick dna viral kit zymo research cat
Quick Dna Viral Kit Zymo Research Cat, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/zymo+cat/Quick-DNA+Viral+Kit/pm42034057-255-231-234
Average 95 stars, based on 188 article reviews

quick dna viral kit zymo research cat - by Bioz Stars, 2026-10

95/100 stars

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Plasmid Preparation:

Article Title: Recombinant arterivirus replicon systems and uses thereof
Article Snippet: Plasmid DNA templates were purified (Qiagen Cat. no. 12163) from 300 mL of saturated E. coli TransforMax Epi300 (Epicentre Cat. no. EC300105) cultures grown in LB broth media (Teknova Cat. no. L8000 06) supplemented with 50 ng/ml carbamicilin (Teknova Cat. no. NC9730116). .. Plasmid DNA was linearized by Not-I digestion (New England Biolabs NEB cat. no. R3189S) for one hour at 37° C. Linearized template DNA was then re-purified (Zymo Cat. no. D4003), and analyzed by 0.8% agarose gel (Life Technologies Cat. no. G5018-08) against a commercial 2-log DNA ladder (New England Biolabs, NEB Cat. no. N3200S). ..

Agarose Gel Electrophoresis:

Article Title: Multiplexed, multimodal profiling of the intracellular activity, interactions, and druggability of protein variants using LABEL-seq
Article Snippet: The next day, 2.5 μL recombinant shrimp alkaline phosphatase (NEB cat#M0371S) was added and incubated at 37°C for 2 hrs. .. Digested DNA was run on a 1% agarose gel, extracted (Zymo cat#D4008), and eluted into 30 μL nuclease-free water. .. The gene fragment library was cloned into the linearized vector using a 1:2 molar ratio of vector:insert by combining the following and incubating at 50°C for 1hr: 80ng linearized vector, 95ng gene fragment library, 40 μL NEBuilder® HiFi DNA Assembly Master Mix (NEB cat#E2621L), and nuclease-free water to 80uL.

cDNA Synthesis:

Article Title: Dual Ribosome Profiling reveals metabolic limitations of cancer and stromal cells in the tumor microenvironment
Article Snippet: A pre-adenylated DNA linker was ligated (T4 Rnl2(tr) K227Q; NEB cat. M0351S), and excess linker removed with 5′ Deadenylase and RecJf (NEB cat. M0331S, cat. M0264S). .. Ribosomal RNA was depleted using biotinylated rRNA oligos (Supplementary Data ) and streptavidin beads (Thermo Fisher cat. 65001). cDNA synthesis (SuperScript III; Thermo Fisher cat. 18080051) was followed by purification on an 8% polyacrylamide-urea gel. cDNA was circularized (CircLigase II; Biosearch cat. CL9021K), PCR-amplified (Q5; NEB cat. M0492S), cleaned (Zymo cat. D4013), size-selected on an 8% gel, and quantified by Qubit HS DNA assay. ..

Article Title: Dual Ribosome Profiling reveals metabolic limitations of cancer and stromal cells in the tumor microenvironment.
Article Snippet: A pre-adenylated DNA linker was ligated (T4 Rnl2(tr) K227Q; NEB cat. M0351S), and excess linker removed with 5′ Deadenylase and RecJf (NEB cat. M0331S, cat. M0264S). .. Ribosomal RNAwas depleted using biotinylated rRNAoligos (Supplementary Data 1) and streptavidin beads (Thermo Fisher cat. 65001). cDNA synthesis (SuperScript III; Thermo Fisher cat. 18080051) was followed by purification on an 8% polyacrylamide-urea gel. cDNA was circularized (CircLigase II; Biosearch cat. CL9021K), PCR-amplified (Q5; NEB cat. M0492S), cleaned (Zymo cat. D4013), size-selected on an 8% gel, and quantified by Qubit HS DNA assay. ..

Purification:

Polymerase Chain Reaction:

Incubation:

Article Title: Genetic analyses suggest that larval and adult stages of Lucilia cuprina employ different sensory systems
Article Snippet: .. The reaction (RXN) was ended by adding 1 μl of Proteinase K 10 μg/μl (Zymo, Cat. # D3001-2-20) and incubated for 10 min. .. The RXN mix was then loaded into a 1.5% agarose gel in parallel to uncut gDNA used before as a template (same concentration), electrophoresed at 60 volts for 90 min and visualize using a Gel DocTM EZ System (Bio-Rad, Hercules, CA, USA).

Article Title: Genetic and behavioral analyses suggest that larval and adult stages of Lucilia cuprina employ different sensory systems to detect rotten beef
Article Snippet: .. The reaction (RXN) was ended by adding 1 μl of Proteinase K 10 μg/μl (Zymo, cat. no. D3001-2-20) and incubated for 10 min. .. The RXN mix was then loaded into a 1.5% agarose gel in parallel to uncut gDNA used before as a template (same concentration), electrophoresed at 60 V for 90 min, and visualized using a Gel DocTM EZ System (Bio-Rad, Hercules, CA, USA).

Article Title: Genetic and behavioral analyses suggest that larval and adult stages of Lucilia cuprina employ different sensory systems to detect rotten beef.
Article Snippet: .. The reaction (RXN) was ended by adding 1 μl of Proteinase K 10 μg/μl (Zymo, cat. no. D3001-2-20) and incubated for 10 min. .. The RXN mix was then loaded into a 1.5% agarose gel in parallel to uncut gDNA used before as a template (same concentration), electrophoresed at 60 V for 90 min, and visualized using a Gel DocTM EZ System (Bio-Rad, Hercules, CA, USA).

Article Title: Multiplexed, multimodal profiling of the intracellular activity, interactions, and druggability of protein variants using LABEL-seq
Article Snippet: To estimate background transformants, a vector-only reaction was run in parallel using 80ng linearized vector, 40 μL NEBuilder Master Mix, and water to 80 μL (no gene fragment library). .. After incubation, reactions were cleaned (Zymo catD4014) and eluted into 20 μL nuclease-free water. .. Cleaned reactions were transformed into NEB® 5-alpha Competent E. coli (High Efficiency) (NEB cat#C2987H) per the manufacturer’s instructions.

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